Published on 23.05.13 in Vol 15, No 5 (2013): May
Web-Based Newborn Screening System for Metabolic Diseases: Machine Learning Versus Clinicians
Background: A hospital information system (HIS) that integrates screening data and interpretation of the data is routinely requested by hospitals and parents. However, the accuracy of disease classification may be low because of the disease characteristics and the analytes used for classification.
Objective: The objective of this study is to describe a system that enhanced the neonatal screening system of the Newborn Screening Center at the National Taiwan University Hospital. The system was designed and deployed according to a service-oriented architecture (SOA) framework under the Web services .NET environment. The system consists of sample collection, testing, diagnosis, evaluation, treatment, and follow-up services among collaborating hospitals. To improve the accuracy of newborn screening, machine learning and optimal feature selection mechanisms were investigated for screening newborns for inborn errors of metabolism.
Methods: The framework of the Newborn Screening Hospital Information System (NSHIS) used the embedded Health Level Seven (HL7) standards for data exchanges among heterogeneous platforms integrated by Web services in the C# language. In this study, machine learning classification was used to predict phenylketonuria (PKU), hypermethioninemia, and 3-methylcrotonyl-CoA-carboxylase (3-MCC) deficiency. The classification methods used 347,312 newborn dried blood samples collected at the Center between 2006 and 2011. Of these, 220 newborns had values over the diagnostic cutoffs (positive cases) and 1557 had values that were over the screening cutoffs but did not meet the diagnostic cutoffs (suspected cases). The original 35 analytes and the manifested features were ranked based on F score, then combinations of the top 20 ranked features were selected as input features to support vector machine (SVM) classifiers to obtain optimal feature sets. These feature sets were tested using 5-fold cross-validation and optimal models were generated. The datasets collected in year 2011 were used as predicting cases.
Results: The feature selection strategies were implemented and the optimal markers for PKU, hypermethioninemia, and 3-MCC deficiency were obtained. The results of the machine learning approach were compared with the cutoff scheme. The number of the false positive cases were reduced from 21 to 2 for PKU, from 30 to 10 for hypermethioninemia, and 209 to 46 for 3-MCC deficiency.
Conclusions: This SOA Web service–based newborn screening system can accelerate screening procedures effectively and efficiently. An SVM learning methodology for PKU, hypermethioninemia, and 3-MCC deficiency metabolic diseases classification, including optimal feature selection strategies, is presented. By adopting the results of this study, the number of suspected cases could be reduced dramatically.
J Med Internet Res 2013;15(5):e98
Newborn screening (NBS) using biochemical markers to detect presymptomatic infants with certain congenital conditions has been performed for almost 50 years. The aim of NBS is to provide early treatment to prevent or ameliorate the long-term consequences of the detected condition [- ]. Since the pioneering work by Guthrie [ ], who discovered that phenylketonuria (PKU) could be detected from dried blood spots collected on filter paper and transported to a testing laboratory, dozens of congenital diseases, including metabolic and infectious diseases, can now be detected in NBS programs [ ]. The advent of tandem mass spectrometry (MS/MS) has resulted in a substantial increase in the number of inborn errors of metabolism (IEMs) included in the NBS panel [ ], including amino acid disorders, fatty acid oxidation disorders, and organic acid disorders [ ].
Although MS/MS can detect many metabolic species and many IEMs, the diseases included in the NBS panels vary from country to country. For example, the American College of Clinical Genetics has proposed that 29 core and 25 secondary conditions be screened , whereas only 12 metabolic disorders are included in the German panel [ ]. In Taiwan, the Newborn Screening Center of the National Taiwan University Hospital (NTUH) introduced MS/MS-based screening in 2001 [ ]. Among the diseases that we can screen, PKU (screened by phenylalanine [Phe] level) and homocystinuria (screened by methionine [Met] level) have been included in the official recommended list [ ]. Nevertheless, 3-methylcrotonyl-CoA carboxylase (3-MCC) deficiency, screened by 3-hydroxyisovalerylcarnitine (C5OH) level, has been the most common condition detected in the MS/MS panel [ ].
The sensitivity of MS/MS screening for the 20 to 30 diseases included in the American College of Clinical Genetics screening panel varies among the individual diseases . For example, PKU can be detected by the elevation of Phe levels and low or normal tyrosine (Tyr) levels. A timely and highly discriminating method, such as MS/MS, provides better performance than the previously used bacterial inhibitory method [ ]. In contrast, Met is a less reliable marker for homocystinuria. Elevation of Met (hypermethioninemia) can occur in various conditions, such as methionine adenosyltransferase deficiency, glycine N-methyltransferase deficiency, S-adenosylhomocysteine hydrolase deficiency, and cystathionine beta-synthase deficiency (classical homocystinuria) [ ], and transient elevation sometimes occurs with liver disease. Some conditions may be benign, with no treatment currently recommended. However, there is an estimated 20% false negative rate in homocystinuria neonatal screening [ ], and the contributing factors include early discharge from hospital, low protein intake from breast-feeding, and pyridoxine responsiveness. Elevation of C5OH indicates 3-MCC deficiency and several related disorders; in addition, some newborns born to a 3-MCC-deficient mother can also test positive. The majority of patients with 3-MCC deficiency will not develop any signs or symptoms of disease. Therefore, a suitable interpretation and prediction method to decrease false positives and avoid false negatives is crucial for a newborn screening program.
To improve the specificity of screening, there are several approaches. The cutoff scheme has been a popular screening method [- ]. Two-tier testing and the use of multiple markers can improve sensitivity and specificity [ ]. For example, more specific markers, such as total homocysteine, methylmalonic acid, and isovalerylglycine, can be detected in dried blood spots (DBSs) and have reduced the false-positive rate and improved the positive predictive value of NBS for different diseases [ , ]. We also tested the possibility of applying molecular second-tier testing for citrin deficiency and carnitine uptake defects [ ]. However, a different testing format is required that is expensive and time consuming. The interpretive tool developed by the Region 4 Genetics Collaborative may help us to enhance the predictive value and minimize the false-positive rate while retaining sensitivity [ ]. Machine learning techniques offer another obvious and promising approach for the examination of high dimensional data. Thus, the goal of this paper is to describe feature selection strategies and use support vector machine (SVM) learning techniques to establish the classification models for metabolic disorder screening and diagnoses.
The NTUH initiated newborn screening research in 1981 and has performed the nation’s newborn screening of metabolic diseases since July 1985. The NTUH simultaneously holds the responsibilities for national NBS, a phlebotomy hospital, and a referral hospital. The entire screening process workflow and the corresponding functions, relationships, and roles of participants are illustrated in. At present, the NTUH Newborn Screening Center provides these services for approximately one-third of the nation’s newborns. Currently, the coverage rate for NBS has improved to 99.9% in the past few years [ ], and the NTUH Newborn Screening Center tests more than 70,000 babies every year.
The overall architecture of the NTUH Newborn Screening Hospital Information System (NSHIS) is depicted in. In this diagram, 3 major components, ie, the front-end module, the middleware module, and the back-end services including the database servers are shown. The front-end module handles user interfaces via Web browsers [ ] and establishes the users’ sessions with the authentication server. The server validates users’ authentications and authorizations. The middleware module, ie, the Health Level Seven (HL7) middleware framework [ ] indicated in the diagram, connects the front-end applications and the back-end facilities. It provides communication and connectivity via a service-oriented architecture (SOA; Web Services) mechanism in the .NET environment. The HL7-embedded Extensible Markup Language (XML)-formatted data are used in the framework for data exchanges among the modules over the simple object access protocol (SOAP) [ , ] and are described in Universal Description, Discovery, and Integration (UDDI) specification with the Web Service Description Language (WSDL). The back-end facilities support services and database storage. The portal server supports the login process with single sign-on service (SSOS) features [ ]. The Web user interface server in the architecture generates Web-based pages for users’ interactive activities, including the newborn screening system user interfaces.
In addition, the HL7 middleware framework performs data synchronization between the NSHIS and the NTUH hospital information system (HIS). The NTUH HIS integrates (1) patient demographic data, (2) patient radiology orders in the radiology information system (RIS) database involving the picture archiving and communication system (PACS), and (3) laboratory orders in a laboratory information system (LIS) to ensure data consistency and integrity across the NSHIS and HIS architecture as indicated in the back-end facilities [- ].
To increase the performance of the NTUH HIS, a cluster of identical servers are deployed and dispatched dynamically by introducing layer 4 (L4) and layer 2 (L2) switches. All the servers are configured to run using load balancing, including failover modes to secure the system’s availability and concurrency. Firewalls are also installed to enhance the security of the architecture.
The NSHIS system is accessible to all authorized screening program professionals and hospitals by enabling the unique identification of babies via screening samples and displaying the results. Authorized users can be doctors, medical staff, administrative personnel, and neonatal parents. The screening hospitals include the Newborn Screening Center, referral hospitals, and phlebotomy clinics as shown on the left in. In other words, these members can access the subsystems of NSHIS for services related to their duties, following the newborn screening procedures and after authentication and authorization by the authentication server.
The data used in this paper were gathered from the Newborn Screening Center of NTUH between 2006 and 2011 (N=347,312). Dried blood samples from 3-day-old newborns were analyzed by MS/MS in a high-throughput process. The measured metabolic properties (35 measured metabolites including amino acids and acylcarnitines) were archived in the NTUH database. In this study, 3 metabolic diseases (PKU, hypermethioninemia, and 3-MCC deficiency) were reanalyzed. Both screening cutoffs and diagnostic cutoffs were applied in this study. Newborns with an initial screening value that exceeded the diagnostic cutoffs were classified as positive cases and were requested to participate in a confirmation test at our hospital. Newborns with an initial screening value not exceeding the diagnostic cutoff, but equal to or exceeding the screening cutoff, were classified as suspected cases and were asked to undergo another DBS screening. The cutoff values for each disease, the numbers of suspected cases and positive cases observed during this period are summarized in. During this period, we did not have any reports of false negatives for these 3 diseases.
|Disease||Screen markers||Suspected cases||Positive cases||Screening cutoff||Diagnostic cutoff|
|PKU||Phe||203||38||> 85.02 μM||> 220 μM|
|Hypermethioninemia||Met||261||40||> 54.12 μM||> 110 μM|
|3-MCC deficiency||C5OH||1093||142||> 0.56 μM||> 2.2 μM|
Feature Selection Strategies
Support Vector Machines
An SVM [, ] performs classification by constructing an N-dimensional hyperplane that optimally separates the data into 2 categories, as shown in . In the parlance of the SVM literature, an attribute is a predictor variable, and a feature is a transformed attribute that is used to define the hyperplane. The task of choosing the most suitable representation is known as feature selection. A set of features that describes 1 case (ie, a row of predictor values) is called a vector. Therefore, the goal of SVM modeling is to find the optimal hyperplane that separates clusters of vectors. Several different kernel functions can be used, such as linear, polynomial, radial basis function (RBF), and sigmoid. The detailed descriptions of the SVM methodology are presented in [ ]. In this study, the machine learning approach only used the SVM RBF kernel. There are 2 input parameters to an SVM: the slack variable (C) is set as default value 100, and the gamma value is set by default to the reciprocal of the number of input features.
Data Training and Prediction
In this study, a properly supervised classification dataflow is proposed to enhance the accuracy and sensitivity of the NBS process, as depicted in. In the diagram, the training data are used to produce the SVM prediction model. Next, the testing data uses the same methods to obtain the prediction result according to the trained model. Before training or predicting, the dataset is preprocessed by the MS/MS machine and digitization procedures. Feature selection generates the most relevant features.
For classifications, the data are divided into the training, testing, and prediction datasets. In the diagram, the strategies are illustrated step-by-step as follows:
The MS/MS data from positive cases and from suspect cases were used as training (step 3) and testing (step 4) datasets. For each analyte, the difference of the median values between the positive cases and the suspect cases is represented as D, as in. The primary weight for each analyte was calculated according to the formula defined in .
The highest 3 positive primary weights of the individual diseases among the 35 analytes were (1) PKU: Phe, stearoylcarnitine (C18), and octadecenoylcarnitine (C18:1); (2) 3-MCC deficiency: C5OH, ornithine (Orn), and arginine (Arg); and (3) hypermethioninemia: octenoylcarnitine (C8:1) and Met. In hypermethioninemia, only 2 analytes showed positive primary weights. The highest 3 negative primary weights of the individual diseases among the 35 analytes were (1) PKU: Arg, leucine (Leu), and Tyr; (2) 3-MCC deficiency: C16, C4, and C16:1; and (3) hypermethioninemia: C16, C16:1, and C12.
The manifested features are generated by combinations of the highest 3 positives and the highest 3 negatives as listed in. The ratios of the highest 3 positives/negatives are also listed in . presents the feature selection strategies used to construct the manifested features via the relevant features of the 3 diseases.
The original 35 analytes and the manifested features were ranked according to the F score indicator. The values of the F score represent the importance of the features. The top 20 ranked features with the highest F scores were selected as the input features. Different combinations of the features, ie, C(20, 1), C(20, 2), C(20, 3),..., C(20, 20), were inputted into the SVM RBF classifier and used to generate the classification model for each combination. Each combination of the features was used as input for the classification models to calculate the sensitivity and specificity. The optimal feature set was defined as the combination with the highest specificity and 100% sensitivity.
The optimal feature set is then fed into the SVM RBF classifier with 5-fold cross-validation and the optimal model is obtained from the 5 different models of each disease with the highest specificity and 100% sensitivity.
Based on the optimal model with the optimal feature set, we provided the NBS data collected in 2011 to be used for prediction. The results were classified as either true positive, true negative, false positive, or false negative. The definition of cases was based on the current method and the confirmation results; therefore, we assumed no false negatives were revealed by the current method.
Newborn Screening Hospital Information System
A new generation of the NSHIS at the Newborn Screening Center of the NTUH was designed and deployed under a SOA Web services middleware framework. The framework applied embedded HL7 standards in SOAP messages to share data among heterogeneous platforms. The NSHIS successfully provides secure, Web-based, real-time, newborn screening applications in Taiwan.
Optimal Feature Sets
According to the described feature selection strategies, the optimal selected markers, ie, optimal feature sets, of the 3 diseases are summarized in. After following step 4 as described previously, the optimal model for each disease is generated.
Of the 347,312 newborn samples collected through the Newborn Screening Center of NTUH from 2006 to 2011, 220 were positive cases and 1557 were suspected cases for the 3 conditions. Because it was a retrospective analysis study, we can only compare the proposed method to the current method to determine if the proposed method has better discriminatory power. After obtaining the optimal model for individual disease from 2006 to 2010, we attempted to predict the disease state for the newborn samples collected in year 2011; the prediction results are listed in.
In, the proposed method receives the same sensitivity as that of the cutoff scheme, ie, 100%, for the 3 diseases. Similarly, for specificity and the accuracy, the proposed approach and the cutoff scheme achieve a sensitivity greater than 99% for the 3 diseases in the current experiments.
The effectiveness of the classifier is shown in. No false positive cases were generated using the proposed approach. Using the proposed approach can significantly decrease the false positive cases for PKU, hypermethioninemia, and especially 3-MCC deficiency.
|PKU||Ala; Met; Phe; C4; C16:1; Leu×Tyr|
|Hypermethioninemia||Arg; Met; Phe; Val; C4; C8; C10; C10:1; C14; C14:1|
|3-MCC deficiency||C3; C5OH; C6; C8; C10:1; C14; C14:1; C16×C4; C4×C16:1; Orn/C16|
aAla: alanine; Met: methionine; Phe: phenylalanine; Leu: leucine; Tyr: tyrosine; Val: valine; Orn: ornithine; Arg: Arginine;C3: propionylcarnitine; C4: isobutyrylcarnitine; C5OH: 3-hydroxyisovalerylcarnitine; C6: hexanoylcarnitine; C8: octanoylcarnitine; C10: decanoylcarnitine; C10:1: decenoylcarnitine; C14: tetradecanoylcarnitine; C14:1: tetradecenoylcarnitine; C16: palmitoylcarnitine; C16:1: palmitoleylcarnitine.
|Diseases||Methods||Sensitivity (%)||Specificity (%)||Accuracy (%)|
|Diseases||Methods||True positives (n)||True negatives (n)||False positives (n)||False negatives (n)|
The Newborn Screening Hospital Information System
The NSHIS is a newborn screening information management system providing services and applications to Newborn Screening Center referral hospitals, phlebotomy clinics, and neonatal parents. The system was designed, developed, and deployed based upon middleware, using SOA Web services loosely coupled to technologies in a .NET environment in C# programming language. The HL7 embedded XML formatted data are used in the system for data exchange among the modules over a SOAP request/response mechanism. This system can integrate diverse platforms and databases (eg, NSHIS and the NTUH HIS), and merge, extend, and enhance the accuracy and the reliability of the proposed screening applications. The functionalities include specimen receiving, specimen tracking, uploading of the testing results, screening data management, quality control analyses, classifications, and Web integration. Undoubtedly, the system can provide timely delivery of complete and accurate information for newborn screening. Therefore, the system is able to comprehensively improve the quality of care and well-being of newborns. A scenario for the NSHIS functionalities is presented in.
The purpose of this research is to separate apparently healthy individuals who have a disease from those who most likely do not. In addition to the original 35 analytes, we also used different mathematical combinations of the analytes for the manifested features. The use of SVM methods contributes to the credibility of the examination and screening results. During the screening process, the false positive cases will be requested to repeat DBS screening or have a confirmation test. Therefore, these cases will consume additional medical resources and increase parents’ anxiety. By adapting this approach, the number of suspected cases can be reduced substantially; additionally, medical resources will be used effectively and efficiently.
The Mayo Clinic College of Medicine cooperated and collaborated globally to establish a database of unprecedented size containing true positive cases . Based on this database and multivariate pattern-recognition software and through the use of postanalytical and interpretive tools, multiple clinically significant results were compiled into a single score [ ]. We also tested these tools and confirmed that they can significantly reduce the false positive cases and eliminate at least half of the cost resulting from unnecessary tests. Although we used only regional data to train our model, after combining our feature selection strategies and the ranked manifested markers, we can estimate the covariance among analytes and decrease the false positive cases. Therefore, we provide another approach to enhance NBS results, although further comparisons using the approach and revisions will be necessary.
The feature selection strategies are initially designed based on mathematical expressions containing the highest 3 positive and negative ranked features to generate the manifested features. The manifested features and the original 35 analytes constitute the total features, for example, PKU has 52 features, whereas hypermethioninemia has 46 features and 3-MCC deficiency has 52 features. Among the total features, the top 20 F score–ranked features are selected for the total combinations as input features for training in order to create optimal feature sets for the 3 diseases. Apparently, the feature selection strategies did not include the total features for establishing either the manifested features or total combinations. To consider all of the features, additional human and computational resources are required to enhance the current automated programming methods.
Presently, PKU, hypermethioninemia, and 3-MCC deficiency are the emphasized metabolic diseases. Other diseases were considered; however, several diseases present problems: (1) carnitine transporter defect and citrullinemia cannot be fully detected using blood samples at birth , and (2) other diseases, such as medium-chain acyl-CoA dehydrogenase deficiency, cannot be evaluated using the proposed approach because of the low number of training cases.
The NSHIS plans to promote comprehensive care by establishing additional applications for home follow-ups and working with the children with the rare inherited disorders and their families . The exact applications are under evaluation and investigation. Recently, the system has been improved and enhanced to include online billing and charging facilities at phlebotomy clinics and referral hospitals. In addition, the newborn screening test for severe combined immunodeficiency (SCID) has been added to the system. Therefore, the development and deployment of the NTUH NSHIS is evolving as needed.
To assist the research group in exploring further feature selection strategies and machine learning algorithms and in improving classification accuracy, the Computer Center of National Taiwan University offers high-performance computing services. The group members can apply for accounts and use the facilities for both programming and computing resources.
A new generation of the NSHIS of the Newborn Screening Center at NTUH has been designed and deployed using a SOA middleware framework. The framework applies embedded HL7 standards in SOAP request/response for data exchanges among heterogeneous platforms integrated by Web Services. We have established SVM RBF classifiers using the experimental datasets of 35 original MS/MS analytes and the manifested features. The system supports a set of new methods to refine the screening statistics. The methodology we demonstrated here can effectively enhance screening accuracy and the quality controls without changing the current screening method. The methodology can be easily adapted for routine MS/MS newborn screening. Although we will need other true positive cases to train and define the features for other diseases, we demonstrate the superiority of SVM in this study.
The authors would like to acknowledge partial funding support from Microsoft Research Asia and assistance from their staff. In addition, the authors thank Professor Chih-Jen Lin and his research team members for kindly providing the LIBSVM tool. F Lai received grants from National Science Council, Taiwan (NSC101-2219-E-002-024) and Ministry of Education, Taiwan (102R7608-3). The funding organizations were not involved in designing or conducting the study, data collection, management, analysis, or interpretation, nor the preparation, review, and approval of the manuscript.
Conflicts of Interest
Multimedia Appendix 1
Newborn screening data workflow process.PDF File (Adobe PDF File), 658KB
Multimedia Appendix 2
Newborn screening for phenylketonuria: machine learning vs clinicians.PDF File (Adobe PDF File), 397KB
Multimedia Appendix 3
NSHIS implementation and a scenario.PDF File (Adobe PDF File), 218KB
- Newborn Screening Tests. URL: http://kidshealth.org/parent/system/medical/newborn_screening_tests.html [accessed 2012-11-26] [WebCite Cache]
- The Children's Hospital at Westmead. The NSW Newborn Screening Programme. 2012. URL: http://www.chw.edu.au/prof/services/newborn/ [accessed 2012-11-26] [WebCite Cache]
- Bodamer OA, Hoffmann GF, Lindner M. Expanded newborn screening in Europe 2007. J Inherit Metab Dis 2007 Aug;30(4):439-444. [CrossRef] [Medline]
- Guthrie R. Blood screening for phenylketonuria. JAMA 1961 Nov 1961;178(8):863. [CrossRef]
- Therrell BL. U.S. newborn screening policy dilemmas for the twenty-first century. Mol Genet Metab 2001;74(1-2):64-74. [CrossRef] [Medline]
- Huang HP, Chu KL, Chien YH, Wei ML, Wu ST, Wang SF, et al. Tandem mass neonatal screening in Taiwan--report from one center. J Formos Med Assoc 2006 Nov;105(11):882-886. [CrossRef] [Medline]
- Millington DS, Stevens RD. Acylcarnitines: analysis in plasma and whole blood using tandem mass spectrometry. Methods Mol Biol 2011;708:55-72. [CrossRef] [Medline]
- American College of Medical Genetics Newborn Screening Expert Group. Newborn screening: toward a uniform screening panel and system--executive summary. Pediatrics 2006 May;117(5 Pt 2):S296-S307 [FREE Full text] [CrossRef] [Medline]
- Lindner M, Gramer G, Haege G, Fang-Hoffmann J, Schwab KO, Tacke U, et al. Efficacy and outcome of expanded newborn screening for metabolic diseases--report of 10 years from South-West Germany. Orphanet J Rare Dis 2011;6:44 [FREE Full text] [CrossRef] [Medline]
- Niu DM, Chien YH, Chiang CC, Ho HC, Hwu WL, Kao SM, et al. Nationwide survey of extended newborn screening by tandem mass spectrometry in Taiwan. J Inherit Metab Dis 2010 Oct;33(Suppl 2):S295-S305. [CrossRef] [Medline]
- McHugh D, Cameron CA, Abdenur JE, Abdulrahman M, Adair O, Al Nuaimi SA, et al. Clinical validation of cutoff target ranges in newborn screening of metabolic disorders by tandem mass spectrometry: a worldwide collaborative project. Genet Med 2011 Mar;13(3):230-254. [CrossRef] [Medline]
- Bjursell MK, Blom HJ, Cayuela JA, Engvall ML, Lesko N, Balasubramaniam S, et al. Adenosine kinase deficiency disrupts the methionine cycle and causes hypermethioninemia, encephalopathy, and abnormal liver function. Am J Hum Genet 2011 Oct 7;89(4):507-515 [FREE Full text] [CrossRef] [Medline]
- Naughten ER, Yap S, Mayne PD. Newborn screening for homocystinuria: Irish and world experience. Eur J Pediatr 1998 Apr;157 Suppl 2:S84-S87. [Medline]
- Ohkubo S, Shimozawa K, Matsumoto M, Kitagawa T. Analysis of blood spot 17 alpha-hydroxyprogesterone concentration in premature infants--proposal for cut-off limits in screening for congenital adrenal hyperplasia. Acta Paediatr Jpn 1992 Apr;34(2):126-133. [Medline]
- Lott JA, Sardovia-Iyer M, Speakman KS, Lee KK. Age-dependent cutoff values in screening newborns for hypothyroidism. Clin Biochem 2004 Sep;37(9):791-797. [CrossRef] [Medline]
- Lindner M, Ho S, Fang-Hoffmann J, Hoffmann GF, Kölker S. Neonatal screening for glutaric aciduria type I: strategies to proceed. J Inherit Metab Dis 2006;29(2-3):378-382. [CrossRef] [Medline]
- Matern D, Tortorelli S, Oglesbee D, Gavrilov D, Rinaldo P. Reduction of the false-positive rate in newborn screening by implementation of MS/MS-based second-tier tests: the Mayo Clinic experience (2004-2007). J Inherit Metab Dis 2007 Aug;30(4):585-592. [CrossRef] [Medline]
- Turgeon CT, Magera MJ, Cuthbert CD, Loken PR, Gavrilov DK, Tortorelli S, et al. Determination of total homocysteine, methylmalonic acid, and 2-methylcitric acid in dried blood spots by tandem mass spectrometry. Clin Chem 2010 Nov;56(11):1686-1695 [FREE Full text] [CrossRef] [Medline]
- Shigematsu Y, Hata I, Tajima G. Useful second-tier tests in expanded newborn screening of isovaleric acidemia and methylmalonic aciduria. J Inherit Metab Dis 2010 Oct;33(Suppl 2):S283-S288. [CrossRef] [Medline]
- Wang LY, Chen NI, Chen PW, Chiang SC, Hwu WL, Lee NC, et al. Newborn screening for citrin deficiency and carnitine uptake defect using second-tier molecular tests. BMC Med Genet 2013;14:24 [FREE Full text] [CrossRef] [Medline]
- Marquardt G, Currier R, McHugh DM, Gavrilov D, Magera MJ, Matern D, et al. Enhanced interpretation of newborn screening results without analyte cutoff values. Genet Med 2012 Jul;14(7):648-655. [CrossRef] [Medline]
- NTUH Newborn Screening Center. Newborn Screening Items. 2012. URL: http://www.ntuh.gov.tw/gene/nbsc/screen/item.html [accessed 2012-12-02] [WebCite Cache]
- Teodoro D, Pasche E, Gobeill J, Emonet S, Ruch P, Lovis C. Building a transnational biosurveillance network using semantic web technologies: requirements, design, and preliminary evaluation. J Med Internet Res 2012;14(3):e73 [FREE Full text] [CrossRef] [Medline]
- Ko LF, Lin JC, Chen CH, Chang JS, Lai F, Hsu KP, et al. HL7 middleware framework for healthcare information system. 2006 Presented at: 8th International Conference on e-Health Networking, Applications and Services (HEALTHCOM), 2006; Aug 17-19, 2006; New Delhi, India p. 17-19.
- Yang TH, Cheng PH, Yang CH, Lai F, Chen CL, Lee HH, et al. A Scalable Multi-tier Architecture for the National Taiwan University Hospital Information System based on HL7 Standard. 2006 Presented at: 19th IEEE International Symposium on Computer-Based Medical Systems (CBMS), 2006; Jun 22-23, 2006; Salt Lake City, UT p. 99-104.
- Phan C. Service Oriented Architecture (SOA) - Security Challenges and Mitigation Strategies. 2007 Presented at: Military Communications Conference, 2007; Oct 29-31, 2007; Orlando, FL p. 1-7.
- Cheng PH, Yang TH, Chen HS, Hsu KP, Chen SJ, Lai JS. Codesign of a healthcare enterprise information portal and hospital information systems. 2005 Presented at: Proceedings of 7th International Workshop on Enterprise networking and Computing in Healthcare Industry, 2005; Jun 23-25, 2005; Busan, South Korea p. 446-449.
- Hsieh SL, Feipei L, Cheng PH, Chen JL, Lee HH, Tsai WN, et al. An Integrated Healthcare Enterprise Information Portal and Healthcare Information System Framework. 2006 Presented at: 28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBS), 2006; Aug 30-Sep 3, 2006; New York City, NY p. 4731-4734.
- Hsieh SH, Hsieh SL, Weng YC, Yang TH, Feipei L, Cheng PH, et al. Middleware based Inpatient Healthcare Information System. 2007 Presented at: Proceedings of the 7th IEEE International Conference on Bioinformatics and Bioengineering (BIBE), 2007; Oct 14-17, 2007; Boston, MA p. 1230-1234.
- Weng YC, Hsieh SL, Hsieh SH, Lai F. Design and Enhance a Dynamic Healthcare Portal Site. 2007 Presented at: IEEE/WIC/ACM International Conferences on Web Intelligence and Intelligent Agent Technology Workshops, 2007; Nov 5-12, 2007; Silicon Valley, CA p. 173-176.
- Chen YW, Lin CJ. Combining SVMs with various feature selection strategies. In: Guyon I, editor. Feature Extraction: Foundations and Applications (Studies in Fuzziness and Soft Computing). The Netherlands: Springer Berlin Heidelberg; 2006:315-324.
- Akay MF. Support vector machines combined with feature selection for breast cancer diagnosis. Expert Systems with Applications 2009 Mar;36(2):3240-3247. [CrossRef]
- Chen WH, Chen HP, Tseng YJ, Hsu KP, Hsieh SL, Chien YH, et al. Newborn Screening for Phenylketonuria: Machine Learning vs Clinicians. 2012 Presented at: The 2012 IEEE/ACM International Conference on Advances in Social Networks Analysis and Mining; Aug 26-29, 2012; Istanbul, Turkey.
- American Academy of Pediatrics. Newborn Screening Overview. 2010. URL: http://www.medicalhomeinfo.org/how/clinical_care/newborn_screening.aspx [accessed 2012-12-07] [WebCite Cache]
|3-MCC deficiency: 3-methylcrotonyl-CoA-carboxylase deficiency|
|DBS: dried blood spot|
|HIS: hospital information system|
|HL7: Health Level Seven|
|IEM: inborn error of metabolism|
|LIS: laboratory information system|
|MS/MS: tandem mass spectrometry|
|NBS: newborn screening|
|NSHIS: Newborn Screening Hospital Information System|
|NTUH: National Taiwan University Hospital|
|PACS: picture archiving and communication system|
|RBF: radial basis function|
|RIS: radiology information system|
|SCID: severe combined immunodeficiency|
|SOA: service-oriented architecture|
|SOAP: simple object access protocol|
|SSOS: single sign-on service|
|SVM: support vector machine|
|UDDI: Universal Description, Discovery, and Integration|
|WSDL: Web Service Description Language|
|XML: Extensible Markup Language|
Edited by G Eysenbach; submitted 15.12.12; peer-reviewed by O Bodamer, A Tirado-Ramos; comments to author 22.01.13; revised version received 04.03.13; accepted 07.04.13; published 23.05.13
©Wei-Hsin Chen, Sheau-Ling Hsieh, Kai-Ping Hsu, Han-Ping Chen, Xing-Yu Su, Yi-Ju Tseng, Yin-Hsiu Chien, Wuh-Liang Hwu, Feipei Lai. Originally published in the Journal of Medical Internet Research (http://www.jmir.org), 23.05.2013.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work, first published in the Journal of Medical Internet Research, is properly cited. The complete bibliographic information, a link to the original publication on http://www.jmir.org/, as well as this copyright and license information must be included.